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Comparison of haemostatic function of PAS-C-platelets vs. plasma-platelets in reconstituted whole blood using impedance aggregometry and thromboelastography.
van Hout, F M A; Bontekoe, I J; de Laleijne, L A E; Kerkhoffs, J-L; de Korte, D; Eikenboom, J; van der Bom, J G; van der Meer, P F.
Affiliation
  • van Hout FMA; Center for Clinical Transfusion Research, Sanquin/LUMC, Leiden, The Netherlands.
  • Bontekoe IJ; Department of Clinical Epidemiology, LUMC, Leiden, The Netherlands.
  • de Laleijne LAE; Department Product and Process Development, Sanquin, Amsterdam, The Netherlands.
  • Kerkhoffs JL; Department Product and Process Development, Sanquin, Amsterdam, The Netherlands.
  • de Korte D; Center for Clinical Transfusion Research, Sanquin/LUMC, Leiden, The Netherlands.
  • Eikenboom J; Department Product and Process Development, Sanquin, Amsterdam, The Netherlands.
  • van der Bom JG; Department of Thrombosis and Hemostasis, LUMC, Leiden, The Netherlands.
  • van der Meer PF; Center for Clinical Transfusion Research, Sanquin/LUMC, Leiden, The Netherlands.
Vox Sang ; 112(6): 549-556, 2017 Aug.
Article in En | MEDLINE | ID: mdl-28597485
BACKGROUND AND OBJECTIVES: There are concerns about the haemostatic function of platelets stored in platelet additive solution (PAS). Aim of this study was to compare the haemostatic function of PAS-C-platelets to plasma-platelets in reconstituted whole blood. MATERIALS AND METHODS: In our experiment, whole blood was reconstituted with red blood cells, solvent-detergent (SD) plasma and either PAS-C-platelets or plasma-platelets (n = 7) in a physiological ratio. On storage days 2, 5, 8 and 13, the agonist-induced aggregation (multiple electrode aggregometry), clot formation (thromboelastography) and agonist-induced CD62P responsiveness (flow cytometry) were measured. RESULTS: Samples with PAS-C-platelets showed significantly lower aggregation than plasma-platelets when induced with adenosine diphosphate, -6 U (95% confidence interval: -8; -4) or thrombin receptor-activating protein, -15 U (-19; -10). Also when activated with collagen and ristocetin, the PAS-C-platelets showed less aggregation, although not statistically significant. All samples with PAS-C-platelets showed significantly lower agonist-induced CD62P responsiveness than samples with plasma-platelets. However, there was no difference regarding all TEG parameters. CONCLUSION: Our findings demonstrate that the function - aggregation and CD62P responsiveness - of PAS-C-platelets in reconstituted whole blood is inferior to that of plasma-platelets, which may have implications in the setting of massive transfusions.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Platelets / Blood Preservation / Hemostasis Limits: Humans Language: En Journal: Vox Sang Year: 2017 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Platelets / Blood Preservation / Hemostasis Limits: Humans Language: En Journal: Vox Sang Year: 2017 Document type: Article Affiliation country: Country of publication: